7105712

IgG Antibody (B33/20) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

IgG

Concentration

1.0 mg/mL

Applications

Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Immunology

Formulation

PBS with No Preservative

Gene ID (Entrez)

3500

Immunogen

Purified polyclonal human Ig Gamma Chain

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Molecular Weight of Antigen

75 kDa

Clone

B33/20

Dilution

Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.5 - 1.0 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human

Gene Alias

immunoglobulin heavy constant gamma 1 (G1m marker)

Gene Symbols

IGHG

Isotype

IgG1 κ

Purification Method

Protein A or G purified

Test Specificity

Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. Its epitope maps in CH2 domain of Fc region of IgG. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), u (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

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Description

  • IgG Monoclonal specifically detects IgG in Human samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence, CyTOF-ready.