Biotin (Vitamin B7 or Vitamin H) Antibody (BTN/2032R) - Azide and BSA Free, Novus Biologicals™
Rabbit Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Biotin (Vitamin B7 or Vitamin H)
Concentration
1 mg/mL
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), In Situ Hybridization (ISH)
Conjugate
Unconjugated
Host Species
Mouse
Formulation
10mM PBS - BSA free with No Preservative
Isotype
IgG1
Purification Method
Protein A or G purified
Test Specificity
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Clone
BTN/2032R
Dilution
Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.5-1 ug/ml, In-situ Hybridization
Classification
Monoclonal
Form
Purified
Target Species
All species
Immunogen
Biotinylated sheep immunoglobulin
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen
2.4 kDa
Related Products
Description
- Biotin (Vitamin B7 or Vitamin H) Monoclonal specifically detects Biotin (Vitamin B7 or Vitamin H) in All Species samples
- It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin, In-situ Hybridization, CyTOF-ready.