CD64 Monoclonal Antibody (10.1), Super Bright™ 600, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD64
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Alias
AI323638; AV092959; CD64; CD64 antigen; CD64A; Fc fragment of IgG high affinity Ib receptor; Fc fragment of IgG receptor Ia; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc fragment of IgG, high affinity Ib, receptor for (CD64); Fc gamma receptor; Fc gamma receptor Ia; Fc receptor, IgG, high affinity I; Fcg1; Fc-gamma receptor 1; Fc-gamma receptor I A1; Fc-gamma RI; Fc-gamma RIA; FcgammaRI; FcgammaRIa; Fcgr1; FCGR1A; Fcgr1b; FcgRI; FCRI; FLJ18345; high affinity IgG Fc receptor, subunit beta; high affinity immunoglobulin gamma Fc receptor I; high-affinity immunoglobulin gamma Fc receptor I; IGFR1; IgG Fc receptor I; IgG high affinity Fc receptor; IGGHAFC; RP11-196G18.2
Gene Symbols
FCGR1A
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
FCGR1A
Clone
10.1
Applications
Flow Cytometry
Conjugate
Super Bright 600
Host Species
Mouse
Target Species
Human
Gene Accession No.
P12314
Gene ID (Entrez)
2209
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The 10.1 monoclonal antibody reacts with human CD64 (FcRI), a 75 kDa type I transmembrane protein
- CD64 is the high affinity receptor for IgG and is involved in antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, and regulation of cytokine production
- Monocytes and macrophages express CD64, while mature granulocytes and lymphocytes are negative
- Applications Reported: The 10.1 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 10.1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
- This can be used at 5 μL (0.25 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm
- We recommend using a 610/20 bandpass filter
- Please make sure that your instrument is capable of detecting this fluorochrome
- When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat
- SB-4400) to minimize any non-specific polymer interactions
- Please refer to the datasheet for Super Bright Staining Buffer or contact eBioscience Technical Support (tech@ebioscience.com) for more information
- CD64 (FCGR1A) encodes a protein that plays an important role in the immune response
- CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1
- CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.