7139401

Galectin 9 Monoclonal Antibody (RG9-35), FITC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Galectin 9

Concentration

0.5 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

36 kDa beta-galactoside-binding lectin; AA407335; AI194909; AI265545; ecalectin; Gal9; gal-9; galectin 9; Galectin9; galectin-9; HOM HD 21; HUAT; Lectin galactose binding soluble 9 (Galectin-9); lectin, galactose binding, soluble 5; lectin, galactose binding, soluble 9; Lectin, galactose binding, soluble 9 (Galectin-9); lectin, galactoside binding soluble 9; lectin, galactoside-binding, soluble, 9; LGALS35; Lgals5; LGALS9; LGALS9A; tumor antigen HOM-HD-21; UAT; urate transporter/channel; urate transporter/channel protein

Gene Symbols

Lgals9

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Lgals9

Clone

RG9-35

Applications

Flow Cytometry

Conjugate

FITC

Host Species

Rat

Target Species

Mouse

Gene Accession No.

O08573

Gene ID (Entrez)

16859

Isotype

IgG2a κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: This RG9-35 monoclonal antibody recognizes mouse Galectin 9, a member of the S-type lectin family
  • Galectin 9 is widely expressed within a majority of hematopoietic cells with the particularly high expression on CD45R(B220)-intermediate pre-B cells
  • Applications Reported: This RG9-35 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This RG9-35 antibody has been tested by flow cytometric analysis of mouse bone marrow cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824) and protocol
  • Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
  • This may be used at less than or equal to 0.06 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions
  • The protein encoded by this gene is an S-type lectin
  • It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H and RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency
  • Multiple alternatively spliced transcript variants have been found for this gene.