7143920
BRD4 Recombinant Rabbit Monoclonal Antibody (BL-151-6F11), Bethyl Laboratories
Rabbit Recombit Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
BRD4
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Target Species
Human
Gene Accession No.
O60885
Gene ID (Entrez)
23476
Immunogen
Between 1312 and 1362
Primary or Secondary
Primary
Content And Storage
4° C
Gene
BRD4
Clone
BL-151-6F11
Classification
Recombinant Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
BBS with 0.1% BSA and 0.09% sodium azide; pH 8.2
Gene Alias
HUNK1
Gene Symbols
BRD4
Isotype
IgG
Purification Method
Ion-exchange chromatography
Product Type
Antibody
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Description
- The recommended shelf life for this product is 1 year from date of receipt
- Application Note: For ICC, formaldehyde fixation is recommended
- Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells
- For IHC, epitope retrieval with c BRD4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family
- It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus
- It is expressed as 2 isoforms; the longer variant has a C-terminus region with a praline and glutamine-rich domains.BRD4 is a chromatin-binding protein whose expression is induced in response to growth stimuli
- BRD4 acts at different stages of the cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth
- It also has a role in regulating chromosomal dynamics during mitosis
- BRD4 functions as a cellular target for E2 protein of papillomavirus.