beta-Catenin, Rat anti-Human, Clone: mAb-Cat1, Millipore Sigma™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
beta-Catenin
Dilution
Western Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).Dot Blot Analysis: A representative lot detected beta-Catenin in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824). Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
beta-Catenin 1;Catenin beta-1
Gene Symbols
CTNNB1;CTNNB;OK/sw-cl.35;PRO2286
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
This rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region.
Clone
mAb-Cat1
Applications
ChIP Assay, Dot Blot, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Rat
Research Discipline
Epigenetics & Nuclear Function
Formulation
Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene ID (Entrez)
NP_001091679.1
Immunogen
BSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Related Products
Description
- Anti-beta-Catenin, clone mAb-Cat1, Cat
- No
- MABE1828, is a highly specific rat monoclonal antibody that targets beta-catenin 1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunoprecipitation, and Western Blottiing
- Catenin beta-1 (UniProt: P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, OK/SW-cl.35, PRO2286) gene (Gene ID: 1499) in human
- Catenin beta-1 is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation
- In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3 beta that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome
- In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes
- O-glycosylation at serine 23 is reported to decreases its nuclear localization and transcriptional activity
- Wnt/beta-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation
- In ESC beta-catenin is trimethylated on Lysine 49 by Ezh2 methyltransferase and this trimethylated form represses neuronal differentiation genes Sox1 and Sox3
- Mutations in CTNNB1 gene are shown to cause colorectal cancers, medulloblastoma, and ovarian cancers
- (Ref.: Hoffmeyer, K., et al
- (2017)
- Cell Reports 18(12); 2815-2824.