ESET (SETDB1), Mouse anti-Mouse, Clone: F0808, Millipore Sigma™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
ESET (SETDB1)
Dilution
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ESET (SETDB1) in Chromatin Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).Immunoprecipitation Analysis: A representative lot immunoprecipitated ESET (SETDB1) in Immunoprecipitation applications (Matsumura, Y., et. al. (2015). Mol Cell. 60(4):584-96).
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Mouse
Gene Alias
Histone-lysine N-methyltransferase SETDB1;EC: 2.1.1.43;ERG-associated protein with SET domain;SET domain bifurcated 1
Immunogen
His-tagged recombinant fragment corresponding to 213 amino acids from the internal region of murine SETDB1.
Primary or Secondary
Primary
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Clone
F0808
Applications
ChIP Assay, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Epigenetics & Nuclear Function
Formulation
Purified mouse monoclonal antibody IgG1 in PBS with 0.01% sodium azide.
Gene Symbols
Setdb1;Eset;Kiaa0067
Isotype
IgG1 κ
Purification Method
Protein G purified
Test Specificity
Clone F0808 specifically detects Histone-lysine N-methyltransferase SETDB1. It targets an epitope with in the internal region.
Related Products
Description
- Anti-ESET (SETDB1), clone F0808, Cat
- No
- MABE1067, is a highly specific mouse monoclonal antibody that targets Histone-lysine N-methyltransferase SETDB1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting
- Histone-lysine N-methyltransferase SETDB1 (UniProt: O88974; also known as EC: 2.1.1.43, ERG-associated protein with SET domain, ESET, SET domain bifurcated 1, SETDB1) is encoded by the Setdb1 (also known as Eset, Kiaa0067) gene in murine species
- SETDB1 is a ubiquitously expressed histone methyltransferase with multiple zinc-binding sites that specifically trimethylates lysine 9 of histone H3 immediately downstream of transcription start sites marked with H3K4me3 to establish the bivalent domain
- It is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by Krüppel-associated box (KRAB) zinc-finger proteins
- The H3K4/H3K9me3 signature keeps RNA polymerase II (Pol II) paused at the proximal promoter and maintains gene expression at low levels, but in a poised state that is ready for activation in lineage-committed cells
- It functions mainly in euchromatin regions, thereby playing a key role in the silencing of euchromatic genes
- It probably acts by forming a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone lysine 9 trimethylation
- SETDB1 is reported to inhibit adipogenesis and its depletion induces preadipocyte differentiation and adipogenesis in mesenchymal stem cells and preadipocytes
- Expression of Cebpa and Pparg genes that are its key targets of SETDB1 is enhanced when SETDB1 is knocked down in undifferentiated cells
- Murine SETDB1 contains two Tudor domains (aa 257-320 and 347-403) and pre-SET (aa 744-817), SET (aa 820-1282), and post-SET (aa 1291-1307) domains that are required for its methyltransferase activity
- (Ref.: Matsumura, Y., et al
- (2015)
- Mol
- Cell 60(4); 584-596).