CD105 (Endoglin) Monoclonal Antibody (SN6), APC-eFluor™ 780, eBioscience™, Invitrogen™

Description

• Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin
• CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages
• It is suggested that CD105 functions in adhesion and embryonic angiogenesis
• Applications Reported: This SN6 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This SN6 antibody has been pre-diluted and tested by flow cytometric analysis of U937 cell line
• This may be used at 5 μL (0.5 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm)
• Please make sure that your instrument is capable of detecting this fluorochrome
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product No
• 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
• 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
• CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells
• It exists on an O- and N-glycosylated homodimer
• Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker
• CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.