beta Actin Monoclonal Antibody (3H2.E4.E12), Rockland™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
beta Actin
Concentration
1 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Gene Alias
ACT-5; ACTA1; ACTB; actb.L; actb1; actba; actin; actin beta; actin, alpha 1, skeletal muscle; actin, beta; actin, beta 1; actin, beta L homeolog; actin, beta, cytoplasmic; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed; Actx; Atcb; bact; bactin1; bactin1 protein; bactzf; B-ACTZF; beta actin; beta cytoskeletal actin; Beta-actin; Beta-actin-1; BOS_22932; BRWS1; Cap Z; capZ beta; cytoplasmic 1; Cytoplasmic beta-actin; cytoskeletal protein; cytoskeleton actin; E430023M04Rik; gamma actin; gamma non-muscle actin; PS1TP5-binding protein 1; PS1TP5BP1; RCJMB04_4h19; wu:fd18f05; XELAEV_18045052mg
Gene Symbols
ACTB
Isotype
IgG2a λ
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Gene
ACTB
Clone
3H2.E4.E12
Applications
ELISA, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human, Mouse, Porcine, Rabbit, Rat
Gene Accession No.
P29751, P60709, P60710, P60711, Q6QAQ1
Gene ID (Entrez)
100009272, 11461, 414396, 60, 81822
Immunogen
Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with beta-Actin peptide near the N-Terminus followed by hybridoma development.
Primary or Secondary
Primary
Product Type
Antibody
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Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- beta Actin antibody is specific for human and mouse beta Actin protein
- Expected to cross-react with a wide range of species due to sequence homology (human, mouse, rat, swine)
- A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates
- Cross-reactivity with other sources has not been determined
- Beta actin is one of six actin isoforms which have been identified
- Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity
- There are six different actin isoforms in human
- The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton
- Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells
- Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix
- Actins are highly conserved proteins that are involved in cell motility, structure and integrity
- Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting
- Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.