Wnt1 Monoclonal Antibody (13F9), Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This antibody reacts with human and mouse Wnt1 protein
• A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, opossum and rat based on a 100% homology with the immunizing sequence
• Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology
• Cross-reactivity with Wnt1 from other sources has not been determined
• Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal
• The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade
• Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor
• Extensive research has shown that deregulation of these pathways or its components can lead to cancer
• Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form
• At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1
• Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways
• Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis
• Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.