Caspase 12 Polyclonal Antibody, Rockland™

Description

• Store vial at -20°C prior to opening
• Aliquot contents and freeze at -20°C or below for extended storage
• Avoid cycles of freezing and thawing
• Centrifuge product if not completely clear after standing at room temperature
• This product is stable for several weeks at 4°C as an undiluted liquid
• Dilute only prior to immediate use
• This affinity purified antibody is directed against mouse Caspase 12
• A BLAST analysis was used to suggest reactivity with the antigen based on 100% homology with the immunizing sequence
• Three distinct signaling pathways lead to programmed cell death (apoptosis)
• The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved
• The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved
• Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion
• Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis
• Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including -gamma-secretase presenilin and beta-amyloid precursor protein (APP)
• Caspase-12 mediates cytotoxicity induced by amyloid-beta
• Caspase-12 is ubiquitously expressed in mouse tissues.