EBI3 Monoclonal Antibody (V1.4F5.25), Rockland™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
IL-35 EBI3
Concentration
1 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
Gene Alias
cytokine receptor; EBI3; EBI-3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus induced gene 3 (similar to Interleukin-27 beta chain precursor); Epstein-Barr virus-induced gene 3 protein; Epstein-Barr virus-induced gene 3 protein homolog; Il27; IL-27; IL27 p28; IL-27 p28 subunit; IL27 subunit; IL-27 subunit alpha; IL-27 subunit beta; Il27a; IL-27A; IL-27-A; IL27-A; IL27B; IL-27B; IL27p28; IL-27p28; IL30; IL35 subunit; ILN; Interleukin; interleukin 27; interleukin 30; Interleukin-27 subunit alpha; Interleukin-27 subunit beta; Interleukin-30; MGC71873; M-IL-27 (p28); p28; RGD1561420
Gene Symbols
Ebi3, IL27
Isotype
IgG2b
Content And Storage
-20°C, Avoid Freeze/Thaw Cycles
Gene
IL27
Clone
V1.4F5.25
Applications
ELISA, Immunoprecipitation, Western Blot
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Mouse
Gene Accession No.
O35228, Q8K3I6
Gene ID (Entrez)
246779, 50498
Immunogen
Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.
Primary or Secondary
Primary
Product Type
Antibody
Related Products
Description
- Store vial at -20°C prior to opening
- Aliquot contents and freeze at -20°C or below for extended storage
- Avoid cycles of freezing and thawing
- Centrifuge product if not completely clear after standing at room temperature
- This product is stable for several weeks at 4°C as an undiluted liquid
- Dilute only prior to immediate use
- This antibody is specific for mouse EBI3 protein
- A BLAST analysis was used to suggest cross-reactivity with EBI3 from mouse sources based on 100% homology with the immunizing sequence
- Cross-reactivity with EBI-3 from other sources has not been determined
- IL-27 (Interleukin 27) is a cytokine belonging to the IL-12 family, and is a heterodimeric cytokine composed of EBI3 and p28 subunits
- IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines
- IL-27 promotes CD4+ T cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up regulating IL-12R beta2, which in turn suppresses Th2 and Th17 differentiation and proliferation
- IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines
- IL-27 acts by binding to its receptor WSX-1 and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting IL-27 involvement in the regulation of immune processes
- It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet
- In humans, the gene is located on the p arm of chromosome 16.