7191089

Papain Polyclonal Antibody, Biotin, Rockland™

Goat Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

Papain

Applications

ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Conjugate

Biotin

Host Species

Goat

Target Species

Plant

Gene Alias

Carica papaya

Primary or Secondary

Primary

Product Type

Antibody

Concentration

1 mg/mL

Classification

Polyclonal

Form

Lyophilized

Regulatory Status

RUO

Formulation

0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2

Immunogen

Papain [Carica papaya]

Content And Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

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Description

  • Store vial at 4°C prior to restoration
  • For extended storage aliquot contents and freeze at -20°C or below
  • Avoid cycles of freezing and thawing
  • Centrifuge product if not completely clear after standing at room temperature
  • This product is stable for several weeks at 4°C as an undiluted liquid
  • Dilute only prior to immediate use
  • Anti-Papain antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above
  • Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Papain [Carica papaya]
  • Cross reactivity against Papain from other sources may occur but have not been specifically determined
  • Papain is a protease derived from the papaya fruit (Carica papaya)
  • It hydrolyses peptides with broad specificity, but shows a preference for large hydrophobic amino acids in the P2 position
  • It is synthesised as an inactive 40 kDa precursor containing a 107 residue N terminal pro region and a 26 residue signal peptide.