7193260
B7-H7 Monoclonal Antibody (MA57YW), PE-Cyanine7, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
B7-H7
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
B7-H5; B7H7; B7-H7; B7y; HERV-H LTR-associating 2; HERV-H LTR-associating protein 2; HHLA2; Human endogenous retrovirus-H long terminal repeat-associating protein 2
Gene Symbols
HHLA2
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Clone
MA57YW
Applications
Flow Cytometry
Conjugate
PE-Cyanine7
Host Species
Mouse
Target Species
Human
Gene Accession No.
Q9UM44
Gene ID (Entrez)
11148
Immunogen
B7-H7 recombinant protein
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
HHLA2
Related Products
Description
- Description: This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells
- Applications Reported: This MA57YW antibody has been reported for use in flow cytometric analysis
- This MA57YW monoclonal antibody recognizes human B7-H7 on live and paraformaldehyde-fixed cells
- Applications Tested: This MA57YW antibody has been pre-diluted and tested by flow cytometric analysis of the HDLM-2 cell line
- This may be used at 5 μL (0.125 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation
- Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically
- Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser B7-H7 (also known as HHLA2) is a membrane protein with three Ig-like domains (IgV-IgC-IgV), whereas other members of the B7 family generally have only two Ig domains (IgV-IgC)
- B7-H7 is somewhat more closely related to B7-H3 and B7-H4 and shares 10% to 18% amino acid identity and 23% to 33% similarity to B7 family members
- B7-H7 protein is widely expressed in epithelium cells of the gallbladder, breast, kidney, colon, small intestines and placenta
- In the immune system, HHLA2 protein is not constitutively expressed on human monocytes/macrophages
- B7-H7 is widely expressed in cancers, especially in triple negative breast cancer.