7200816

E. Coli J5 LPS Antibody, Novus Biologicals™

Sheep Polyclonal Antibody

Manufacturer: Fischer Scientific

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Antigen

E. Coli J5 LPS

Applications

ELISA

Conjugate

Unconjugated

Host Species

Sheep

Target Species

Bacteria

Gene Alias

Escherichia coli

Isotype

IgG

Purification Method

Unpurified

Test Specificity

NB100-62551 recognizes the J5 mutant of E. coli 0:111 which lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The 55 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).

Dilution

ELISA 1:100-1:2000

Classification

Polyclonal

Form

Antisera

Regulatory Status

RUO

Formulation

Whole antisera with 0.09% Sodium Azide

Immunogen

Heat inactivated Escherichia coli J5 (Rc mutant; ATCC)

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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Description

  • E
  • Coli J5 LPS Polyclonal specifically detects E
  • Coli J5 LPS in Bacteria samples
  • It is validated for ELISA.