CD56 (NCAM) Monoclonal Antibody (TULY56), Alexa Fluor™ 660, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD56 (NCAM)
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
adhesion molecule; antigen recognized by monoclonal antibody 5.1H11; Cd56; CD-56; CD56 120 kDa GPI-linked isoform; CD56 140 kDa isoform; CD56 140 kDa VASE isoform; E NCAM; E-NCAM; MSK39; N CAM1; NCAM; N-CAM; Ncam1; N-CAM-1; NCAM-1; NCAMC; NCAM-C; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM
Gene Symbols
Ncam1
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Ncam1
Clone
TULY56
Applications
Flow Cytometry
Conjugate
Alexa Fluor 660
Host Species
Mouse
Target Species
Human, Non-human Primate, Rhesus Monkey
Gene Accession No.
P13591
Gene ID (Entrez)
4684, 693789
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The TULY56 monoclonal antibody reacts with human CD56, also known as Neural Cell Adhesion Molecule (NCAM)
- CD56 is a highly glycosylated transmembrane molecule expressed by neurons and plays a role in the homotypic adhesion of neural cells
- In the hematopoietic system, CD56 is expressed on NK cells and a subset of T cells referred to as NKT cells
- Staining with TULY56 does not block binding of CMSSB, suggesting that the two antibodies recognize different epitopes
- Additionally, TULY56 performs better after fixation and permeabilization than CMSSB
- The TULY56 monoclonal antibody crossreacts with Rhesus macaque
- Applications Reported: This TULY56 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This TULY56 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
- This may be used at 5 μL (0.125 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700
- Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser CD56 (NCAM, neural cell adhesion molecule) is a transmembrane glycoprotein of the immunoglobulin family that serves as an adhesive molecule and is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa
- CD56 is found on T cells and NK cells, and is involved in cell migration, axonal growth, pathfinding and synaptic plasticity
- Polysialic modification results in reduction of CD56-mediated cell adhesion
- Through its extracellular region, CD56 mediates homophilic and heterophilic interactions by binding extracellular matrix components such as laminin and integrins
- CD56 is expressed on most neuroectodermal derived cell lines, tissues and neoplasms such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma
- Further, CD56 is a widely used neuroendocrine marker with a high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors
- Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms.