CD116 Monoclonal Antibody (4H1), eBioscience™, Invitrogen™

Description

• Description: The 4H1 monoclonal antibody reacts with the human CD116 molecule, the alpha subunit of GM-CSF receptor
• The alpha subunit associates with the common beta chain (CD131) to form the high affinity receptor for GM-CSF
• The GM-CSFR alpha chain is expressed by granulocytes, monocytes, endothelial cells, fibroblasts and some tumor cells
• Applications Reported: 4H1 has been reported for use in flow cytometric analysis
• Applications Tested: The 4H1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• The protein encoded by this gene is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2, a cytokine which controls the production, differentiation, and function of granulocytes and macrophages
• The encoded protein is a member of the cytokine family of receptors
• This gene is found in the pseudoautosomal region (PAR) of the X and Y chromosomes
• Multiple transcript variants encoding different isoforms have been found for this gene, with some of the isoforms being membrane-bound and others being soluble.