SPI-B Monoclonal Antibody (235D), eBioscience™, Invitrogen™

Description

• Description: The 235D monoclonal antibody recognizes human SPI-B
• SPI-B is a member of the ETS transcription factor family which is comprised of DNA binding proteins that function in lymphoid development and activity
• SPI-B binds the PU box, a purine-rich DNA sequence (5'-GAGGAA-3')
• SPI-B is expressed in mature B cells, immature T cells, plasmacytoid dendritic cells, and microfold cells (M cells) of the intestine
• In human tonsil tissue, high levels of SPI-B are expressed in plasmacytoid dendritic cells, while lower levels of expression are found in germinal center B cells
• Applications Reported: This 235D antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, immunohistochemical staining, and immunocytochemistry
• Applications Tested: This 235D antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 5 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Purity: Greater than 90%, as determined by SDS-PAGE
• Aggregation: Less than 10%, as determined by HPLC
• Filtration: 0.2 μm post-manufacturing filtered
• Spi-B is a transcription factor belonging to a subfamily of ETS transcription factors with a conserved ETS domain that mediates specific DNA binding
• Spi-B may form homotypic interactions and is known to interact with IRF4, CREBBP, EP300, SPI1/PU.1 related, JUN and TBP
• As a sequence specific transcriptional activator it binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer
• Two isoforms of Spi-B are reported
• Spi-B is usually expressed in plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells and promotes their development and block viral replication
• Spi-B is not expressed in T-cells or granulocytes but may be present in stem cell populations of the liver and B-cells where it helps in B-cell receptor (BCR) signaling, normal B-cell development and antigenic stimulation.