LIF Polyclonal Antibody, PeproTech®, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
LIF
Applications
ELISA, Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Target Species
Human
Gene Accession No.
P15018
Gene ID (Entrez)
3976
Immunogen
E.coli-derived Recombinant Human LIF
Purification Method
Antigen affinity chromatography
Gene
Lif
Concentration
0.1-1.0 mg/mL
Classification
Polyclonal
Form
Lyophilized
Regulatory Status
RUO
Formulation
PBS with no preservative
Gene Alias
CDF; cholinergic differentiation factor; cholinergic neuronal differentiation factor; d factor; DIA; differentiation inhibitory activity; differentiation-inducing factor; differentiation-stimulating factor; Emfilermin; hepatocyte-stimulating factor III; HILDA; human interleukin in DA cells; leukemia inhibitory factor; leukemia inhibitory factor (cholinergic differentiation factor); LIF; Melanoma-derived LPL inhibitor; MLPLI; myeloid leukaemia inhibitory factor
Gene Symbols
Lif
Primary or Secondary
Primary
Content And Storage
-20°C
Related Products
Description
- AA Sequence of recombinant protein: SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human LIF
- Anti-Human LIF-specific antibody was purified by affinity chromatography employing an immobilized Human LIF matrix
- Sandwich ELISA: To detect Human LIF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required
- This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human LIF (500-P39Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human LIF
- Western Blot: To detect Human LIF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LIF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- Leukemia inhibitory factor (LIF) is a 20 kDa protein that belongs to the IL-6 receptor family
- It binds to a heterodimeric membrane receptor made up of a LIF-specific subunit, gp190 or LIFR, and the subunit gp130, which is shared with the other members of the IL-6 family
- LIF expression has been observed in various tissues including thymus, lung, and neuronal tissue
- LIF can be up-regulated by pro-inflammatory cytokines such as TNFα and IL-17, and elevated levels of LIF have been found in cases of rheumatoid arthritis, neural injury, systemic inflammation, and tuberculosis
- LIF displays diverse biological effects, but is best known for its ability to inhibit the differentiation of embryonic stem cells in mice and contribute to stem cell self-renewal.Human and mouse LIF share 79% sequence homology and exhibit cross-species activity
- However, LIF inhibition of stem cell differentiation appears to be mouse-specific.