7244927
WISP2 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
WISP2
Applications
ELISA, Western Blot
Conjugate
Biotin
Host Species
Rabbit
Target Species
Human
Gene Accession No.
O76076
Gene ID (Entrez)
8839
Immunogen
E.coli-derived Recombinant Human CTGFL/WISP-2
Purification Method
Antigen affinity chromatography
Gene
CCN5
Concentration
0.1-1.0 mg/mL
Classification
Polyclonal
Form
Lyophilized
Regulatory Status
RUO
Formulation
PBS with no preservative
Gene Alias
CCN family member 5; CCN family protein COP-1; Ccn5; connective tissue growth factor-like protein; Connective tissue growth factor-related protein 58; Cop1; Crgr4; CT58; CTGFL; CTGF-L; Rcop1; UNQ228/PRO261; WISP2; WISP-2; WNT1 inducible signaling pathway protein 2; WNT1-inducible-signaling pathway protein 2
Gene Symbols
CCN5
Primary or Secondary
Primary
Content And Storage
-20°C
Related Products
Description
- AA Sequence of recombinant protein: MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF
- Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CTGFL/WISP-2
- Anti-Human CTGFL/WISP-2-specific antibody was purified by affinity chromatography and then biotinylated
- Sandwich ELISA: To detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required
- This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human CTGFL/WISP-2 (500-P212) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hCTGFL/WISP-2
- Western Blot: To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL
- Used in conjunction with compatible secondary reagents the detection limit for Recombinant hCTGFL/WISP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions
- 500-P212BT-1MG will be provided as 2 x 500 μg Wisp2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family
- WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes
- The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain
- Wisp2 lacks the CT domain which is implicated in dimerization and heparin binding
- It is 72% identical to the mouse protein at the amino acid level
- This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation
- Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors
- It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.