78-579-082

IgM Monoclonal Antibody (II/41), Super Bright™ 780, eBioscience™, Invitrogen™

Manufacturer: eBioscience

Select a Size

Pack Size SKU Availability Price
Each of 1 78-579-082-Each-of-1 In Stock ₹ 31,862.00

78-579-082 - Each of 1

₹ 31,862.00

In Stock

Quantity

1

Base Price: ₹ 31,862.00

GST (18%): ₹ 5,735.16

Total Price: ₹ 37,597.16

Antigen

IgM

Classification

Monoclonal

Concentration

0.2 mg/mL

Formulation

PBS with BSA and 0.09% sodium azide; pH 7.2

Gene Accession No.

P01872

Gene Symbols

Ighm

Purification Method

Affinity chromatography

Regulatory Status

RUO

Gene ID (Entrez)

16019

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Form

Liquid

Applications

Flow Cytometry

Clone

II/41

Conjugate

Super Bright 780

Gene

Ighm

Gene Alias

AGM1; AI326478; Igh6; Igh-6; Ighm; Igh-M; Igm; immunoglobulin heavy constant mu; MU; muH; VH

Host Species

Rat

Quantity

100 μg

Primary or Secondary

Primary

Target Species

Mouse

Product Type

Antibody

Isotype

IgG2a κ

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Description

  • Description: The II/41 monoclonal antibody reacts with the mu heavy chain of mouse IgM
  • It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA
  • IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells
  • Fluorochrome conjugated II/41 can be used as a detection secondary for mouse IgM
  • Applications Reported: The II/41 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining, and immunocytochemistry
  • Applications Tested: This II/41 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
  • This can be used at less than or equal to 1.0 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • This II/41 antibody has been tested by immunocytochemistry of fixed cells using a mouse IgM primary antibody
  • This antibody can be used at less than or equal to 10 μg/mL
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm
  • We recommend using a 780/60 bandpass filter
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen
  • It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum
  • Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens
  • It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated
  • Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.