IgD Monoclonal Antibody (IA6-2), Super Bright™ 780, eBioscience™, Invitrogen™

Description

• Description: The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM
• ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family
• Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains
• Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation
• ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues
• Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2
• ISA-3 has the same reactivity pattern and characteristics as F44
• ISA-3 was generated against the human ICOS antigen
• C398.4A, anti-mouse ICOS/H4 (catalog No
• 14-9949), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3
• C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC
• To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948 or 12-9948 rather than 11-9948
• Applications Reported: This ISA-3 antibody has been reported for use in flow cytometric analysis
• This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM
• IgD also exists as a soluble form in blood serum
• Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.