MilliporeSigma™ Rabbit anti-MYPT1, Polyclonal,

Description

• Specifically detects MYPT1 in Human samples, and it is validated for Western Blotting Chicken Protein phosphatase 1 regulatory subunit 12A (also known as PP1M subunit M110, Myosin phosphatase target subunit 1, Myosin phosphatase-targeting subunit 1, Protein phosphatase myosin-binding subunit, Smooth muscle myosin phosphatase myosin-binding subunit, Protein phosphatase 1, regulatory (inhibitor) subunit 12A, 130kDa myosin-binding subunit of smooth muscle myosin phophatase) is encoded by the Gallus gallus PP1R12A (also known as MBS, MBSP, MYPT1) gene (Entrez Gene ID 396020)
• MYTP1 protein is one of the subunits and an integral component of Myosin phosphatase
• Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase
• It is also involved in smooth muscle contraction
• Inhibition of Myosin light chain phosphatase results in Ca 2+ sensitization of smooth muscle contraction
• This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle
• The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20kDa Myosin light chain to MYPT1
• PP1c interacts with at least four binding sites on the amino-terminus of MYPT1
• MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity
• Anti-MYPT1 is raised against GST fustion protein containing recombinant chicken MYPT1 protein C-terminal tail fragment (UniProt Q90623) that is present in both isoform 1 (M133, 1004aa) and isoform 2 (M130, 963aa)
• Human reactivity of this product is confirmed by Western blotting analysis using HeLa cell lysate.