SREBP-1, Rabbit anti-Human, Polyclonal, Millipore Sigma™
Manufacturer: MilliporeSigma
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABS150825UG-Each-of-1 | In Stock | ₹ 17,118.26 |
ABS150825UG - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
SREBP-1
Classification
Polyclonal
Dilution
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected SREBP-1 in human adrenal gland and human kidney tissues.
Gene Alias
Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species
Rabbit
Molecular Weight of Antigen
∽120 kDa observed; 121.67 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Quantity
25 μg
Research Discipline
Signaling
Test Specificity
This rabbit polyclonal antibody detects Sterol regulatory element-binding protein 1 in human.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Applications
Immunohistochemistry (Paraffin), Western Blot
Conjugate
Unconjugated
Formulation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
SREBF1;BHLHD1;SREBP1
Immunogen
KLH-conjugated linenar peptide corresponding to 14 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1. The Immunogen sequence is conserved in SREBP-1A, B, and C and in isoform 4.
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Human
Isotype
IgG
Related Products
Description
- Anti-SREBP-1, Cat
- No
- ABS1508, is a rabbit polyclonal antibdody that detects Sterol regulatory element-binding protein 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting
- Sterol regulatory element-binding protein 1 (UniProt: P36956; also known as SREBP-1, Class D basic helix-loop-helix protein 1, bHLHd1, Sterol regulatory element-binding transcription factor 1) is encoded by the SREBF1 (also known as BHLHD1, SREBP1) gene (Gene ID: 6720) in human
- SREBP-1 is a multi-pass membrane protein that acts as at transcriptional activator required for lipid homeostasis
- Six isoforms of SREBP-1 have been described that are produced by alternative splicing
- SREBP-1 is expressed in a wide variety of tissues and is most abundant in liver and adrenal gland
- In fetal lung and liver it is expressed in high levels
- SREBP-1A predominates in hepatoma cell lines while isoform SREBP-1C is predominantly found in liver, adrenal gland, and ovary
- Both isoforms IA and IC is found in kidney, brain, white fat, and muscle
- SREBP-1 regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway
- It binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') and has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3')
- SREBP-1 has two cytoplasmic domains (aa 1-487 and 567-1147; two helical domains (aa 488-508 and 548-568), and a luminal domain (aa 509-547)
- In the absence of sterols, it moves from the endoplasmic reticulum to the Golgi
- At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER
- In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease
- The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane.