MilliporeSigma™ PDH, Rabbit, Unlabeled, Polyclonal,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | ABS2082MI-Each-of-1 | In Stock | ₹ 38,958.86 |
ABS2082MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,958.86
GST (18%): ₹ 7,012.595
Total Price: ₹ 45,971.455
Antigen
PDH
Classification
Polyclonal
Conjugate
Unconjugated
Gene
PDHA1, PDHA, PDHAD, PHE1A, PDHB, PDHBD, PHE1B
Host Species
Rabbit
Purification Method
Unpurified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This polyclonal antiserum detects both PDH alpha and beta subunits (Pliss, L., et al. (2004). Neurochem. 91(5):1082-1091).
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Concentration
Please refer to lot specific datasheet.
Formulation
Rabbit polyclonal antibody serum with 0.05% Sodium Azide.
Gene Accession No.
P08559;P11177
Immunogen
Co-expressed recombinant full-length mature human PDH E1 alpha and beta subunits.
Quantity
100 μL
Research Discipline
Signaling
Gene ID (Entrez)
NP_000275;NP_000916
Target Species
Human
Form
Serum
Related Products
Description
- Pyruvate dehydrogenase E1 component subunits alpha and beta, mitochondrial (EC 1.2.4.1; UniProt P08559 and P11177; also known as PDHE1-A type I and PDHE1-B, respectively) are encoded by the PDHA1 (also known as PDHA, PDHAD, PHE1A) and the PDHB (also known as PDHBD, PHE1B) genes (Gene ID 5160 and 5162, respectively) in human
- The pyruvate dehydrogenase (PDH) complex catalyzes the overall, irreversible conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle
- This complex contains multiple copies of three enzymatic components: PDH (E1 or PDHE1-A type I), dihydrolipoamide acetyltransferase (E2), and lipoamide dehydrogenase (E3)
- PDH (E1) performs the first two reactions within the PDH complex: a decarboxylation of pyruvate and a reductive acetylation of lipoic acid
- Lipoic acid is covalently bound to dihydrolipoamide acetyltransferase (E2), which is the second catalytic component enzyme of PDC
- The reaction catalyzed by PDH (E1) is considered to be the rate-limiting step for the PDH complex
- PDH is regulated by both pyruvate dehydrogenase kinase (PDK)-mediated phosphorylation and feedback inhibition
- Phosphorylation at Ser-232, Ser-293 and Ser-300 by PDK family kinases inactivates the enzyme; for this phosphorylation at a single site is sufficient
- Dephosphorylation at all three sites, Ser-232, Ser-293, and Ser-300, is required for reactivation
- PDH (E1) deficiency has been linked to primary lactic acidosis in children.