CD90.2 Rat anti-Mouse, BD IMag-DM, Clone: 30-H12, BD

Description

• BD IMag anti-mouse CD90.2 (Thy-1.2) Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces
• These particles are optimized for the positive selection or depletion of CD90.2-bearing leukocytes using the BD IMagnet™
• The CD90.2 alloantigen is expressed on thymocytes, most peripheral T lymphocytes, some intraepithelial T lymphocytes (IEL, DEC), epithelial cells, fibroblasts, neurons, hematopoietic stem cells, but not B lymphocytes, of most mouse strains
• 30-H12 mAb has been reported not to cross-react with mouse Thy-1.1 (e.g., AKR/J, PL), or with rat Thy-1
• Leukocytes are labeled with BD IMag™ anti-mouse CD90.2 Particles - DM according to the Magnetic Labeling Protocol
• his labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat
• No
• 552311)
• Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction)
• The tube is then removed from the magnetic field for resuspension of the positive fraction
• The separation is repeated twice to increase the purity of the positive fraction
• The magnetic separation steps are diagrammed in the Separation Flow Chart
• After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry
• Host Species: Clone: 30-H12 Cell Separation