BDB551539
CD4 Rat anti-Mouse, BD IMag-DM, Clone: GK1.5, BD
Manufacturer: BD Biosciences
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BDB551539-Each-of-1 | In Stock | ₹ 59,808.00 |
BDB551539 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 59,808.00
GST (18%): ₹ 10,765.44
Total Price: ₹ 70,573.44
Antigen
CD4
Classification
Monoclonal
Conjugate
BD IMag-DM
Formulation
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Immunogen
Mouse CTL clone V4
Quantity
10 mL
Research Discipline
Immunology
Target Species
Mouse
Isotype
IgG2b κ
Applications
Flow Cytometry
Clone
GK1.5
Description
Cd4; CD4 antigen; L3T4; Ly-4; T-cell surface antigen T4/Leu-3
Host Species
Rat
Purification Method
Affinity Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C
Related Products
Description
- BD IMag™ anti-mouse CD4 Particles - DM are magnetic nanoparticles that have monoclonal antibody conjugated to their surfaces
- These particles are optimized for the positive selection or depletion of CD4-bearing leukocytes using the BD IMagnet™
- The CD4 (L3T4) differentiation antigen has been reported to be expressed on most thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells
- In addition, CD4 has also been reported to be detectable on pluripotent hematopoietic stem cells, bone marrow myeloid and B-lymphocyte precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells
- Leukocytes are labeled with BD IMag™ anti-mouse CD4 Particles - DM according to the Magnetic Labeling Protocol
- This labeled cell suspension is then placed within the magnetic field of the BD IMagnet™ (Cat.No
- 552311)
- Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off (negative fraction)
- The tube is then removed from the magnetic field for resuspension of the positive fraction
- The separation is repeated twice to increase the purity of the positive fraction
- The magnetic separation steps are diagrammed in the Separation Flow Chart
- After the positive fraction is washed, the small size of the magnetic particles allows the positive fraction to be further evaluated in downstream applications such as flow cytometry.