CD212 Mouse anti-Mouse, PE, Clone: 114, BD

Description

• The 114 monoclonal antibody specifically binds to mouse CD212 (the β 1 subunit of IL-12R β 1), originally termed IL-12R β , of the mouse IL-12 receptor complex
• The IL-12R β 1 subunit associates with a β 2 subunit to form a heterodimeric IL-12 receptor complex
• Each one of the IL-12R subunits exhibits low affinity for IL-12, but in combination, they bind IL-12 with high affinity
• The IL-12R β 1 subunit interacts primarily with IL-12 p40 whereas the IL-12R β β 1 has more recently been described to bind IL-23, a heterodimer formed of the p40 subunit from IL-12, and p19
• The cytoplasmic regions of the β 1 and β 2 subunits contain the box1 and box2 motifs found in other cytokine receptors such as gp130, LIFR and G-CSFR
• IL-12R β 1 are primarily expressed by activated T cells and NK cells
• Experiments with IL-12R β 1 deficient mice have shown that IL-12R β 1 is necessary for mouse T and NK cell responsiveness to IL-12 p75
• The 114 antibody was generated by immunizing IL-12R β 1 deficient mice of (129 x BALB/c)F1 background with mouse Ba/F3 cells that were stably transfected with IL-12R β 1 This antibody is routinely tested by flow cytometric analysis
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.