Bcl-2 Hamster anti-Mouse, FITC, Clone: 3F11, BD

Description

• Programmed cell death (apoptosis) is a normal physiologic process which occurs during embryonic development as well as in maintenence of tissue homeostasis
• The apoptotic program is characterized by certain morphological features
• These include changes in the plasma membrane such as loss of membrane asymmetry and attachment, a condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA
• In the final stages, the dying cells become fragmented into “apoptotic bodies” which are rapidly eliminated by phagocytic cells without eliciting significant inflammatory damage to surrounding cells
• Members of the Bcl-2 family play a major role in regulating the cellular response to apoptotic signals
• Bcl-2 is considered to be a novel proto-oncogene because it blocks apoptosis in many cell types
• Bcl-2 is thought to provide selective survival advantage for cells by blocking apoptosis and thus may contribute to tumorigenesis
• Bcl-2 is a 26 kD intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane
• Clone 3F11 reacts with mouse Bcl-2
• It does not cross-react with human Bcl-2
• Gel purified recombinant mouse Bcl-2 protein was used as immunogen
• Clone A19-3 is an Armenian hamster IgG isotype control which is specific for the hapten trinitrophenol (TNP)
• This hapten is not expressed on human cells or human cell lines
• The solutions are free of unconjugated antibody
• The 3F11 and A19-3 FITC conjugates are matched in F/P ratios
• The optimum F/P ratio was experimentally determined by flow cytometric analysis.