BDB561880
CD45R/B220 Rat anti-Mouse, APC, Clone: RA3-6B2, BD
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BDB561880-Each-of-1 | In Stock | ₹ 10,368.50 |
BDB561880 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 10,368.50
GST (18%): ₹ 1,866.33
Total Price: ₹ 12,234.83
Antigen
CD45R
Classification
Monoclonal
Concentration
0.2mg/mL
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen
Mouse Abelson Leukemia Virus-Induced pre-B tumor cells
Quantity
25 μg
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Applications
Flow Cytometry
Clone
RA3-6B2
Conjugate
APC
Host Species
Rat
Purification Method
Affinity Purified
Regulatory Status
RUO
Target Species
Human, Mouse
Isotype
IgG2a κ
Related Products
Description
- The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues
- It is expressed on B lymphocytes at all stages from pro-B through mature and activated B cell, but it is decreased on plasma cells and a subset of memory B cells
- The levels of CD45R expression on the B-cell lineage appear to be developmentally regulated
- It is also reportedly found on the abnormal T cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC-restricted CTL), on apoptotic T lymphocytes of mice injected with bacterial superantigen, on a population of NK-cell precursors in the bone marrow, and on B-lymphocyte, T-lymphocyte, and macrophage progenitors in fetal liver
- The CD45R antigen has been reported not to be on hematopoietic stem cells, naive T lymphocytes, or MHC-restricted CTL
- CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation
- The CD45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific
- The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction
- CD45R is commonly used as a pan B-cell marker; however, CD19 expression, detectable by the rat anti-mouse CD19 antibody (clone 1D3), is reported to be more restricted to the B-cell lineage
- The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses
- Cross-reaction of the RA3-6B2 clone with activated human T lymphocytes has also been reportedly observed.