Stat5 (pY694) Mouse, PE, Clone: 47/Stat5(pY694), BD

Description

• Stat ( S ignal t ransducer and a ctivators of transcription) proteins are critical mediators of the biologic activity of cytokines, including interleukins, interferons, erythropoietin, and growth factors
• Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of Stat proteins by tyrosine phosphorylation
• Active Stat proteins then move to the nucleus to promote transcription of cytokine-inducible genes
• Seven Stat proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner
• Stat5 has been characterized and shown to be encoded by two separate genes, Stat5a and Stat5b that share over 90% identity at the amino acid level
• Stat5a has been shown to be involved in lactogenesis and mammary development, while Stat5b has been shown to be involved in growth hormone signaling and to play a role in liver gene expression
• Both Stat5a and Stat5b share similarities, both are involved in IL-2 induced peripheral T cell proliferation
• The peptide hormone, prolactin, binds to the prolactin receptor (PRLR) to initiate the lactogenic response
• There are at least three forms of PRLR; however, only the long form is able to activate the 92-kDa Stat5 protein by inducing phosphorylation at Y694
• Once phosphorylated, Stat5 becomes an essential transcription factor which binds to the β-casein gene promoter
• The presence of an SH2 domain within Stat5 suggests that it may directly interact with protein tyrosine kinases (PTKs) such as JAK2
• The 47 monoclonal antibody recognizes the phosphorylated Y694 of Stat5a
• The homologous phosphorylation site in Stat5b is Y699.