BDB563852
CD3 Mouse anti-Human, Brilliant Violet 650, Clone: UCHT1, BD
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BDB563852-Each-of-1 | In Stock | ₹ 39,516.00 |
BDB563852 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 39,516.00
GST (18%): ₹ 7,112.88
Total Price: ₹ 46,628.88
Antigen
CD3
Classification
Monoclonal
Conjugate
Brilliant Violet 650
Formulation
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Immunogen
Human infant thymocytes and peripheral blood lymphocytes from a Sezary Syndrome donor
Quantity
100 Tests
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Applications
Flow Cytometry
Clone
UCHT1
Description
CD3e; CD3E; T3E; TCRE; T-cell surface antigen T3/Leu-4 epsilon
Host Species
Mouse
Purification Method
Affinity Purified
Regulatory Status
RUO
Target Species
Human
Isotype
IgG1 κ
Related Products
Description
- The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex
- CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes
- Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody
- CD3 plays a central role in signal transduction during antigen recognition
- The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε
- The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon™ Brilliant Violet™ family of dyes
- This dye is a tandem fluorochrome of BD Horizon™ BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
- BD Horizon™ BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
- Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
- However, the spillover can be corrected through compensation as with any other dye combination.