CD279 (PD-1) Mouse anti-Human, Brilliant Violet 421, Clone: MIH4, BD

Description

• The MIH4 monoclonal antibody specifically binds to the program death 1 (PD-1) receptor CD279, a member of the Ig superfamily
• CD279 is an immunoregulatory receptor expressed on activated T cells, B cells and myeloid cells
• Mice deficient in CD279 show a breakdown of peripheral tolerance and manifest multiple autoimmune symptoms
• It contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region
• PD-L1 and PD-L2 are ligands of CD279 and are members of the B7 gene family
• Interaction of CD279:PD-Ligands results in inhibition of T cell proliferation and cytokine secretion
• Reports suggest that the B7/CTLA-4 pathway functions primarily to attenuate, limit, and/or terminate naοve T-cell activation in secondary lymphoid organs
• The PD-ligand:CD279 pathway, on the other hand, may primarily attenuate, limit, and/or terminate T-, B-, and myeloid cell activation/effector function at sites of inflammation in the periphery
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.