BDB565786

IgG1 Rat anti-Mouse, Brilliant Violet 711, Clone: A85-1, BD

Manufacturer: BD Biosciences

Select a Size

Pack Size SKU Availability Price
Each of 1 BDB565786-Each-of-1 In Stock ₹ 27,367.50

BDB565786 - Each of 1

₹ 27,367.50

In Stock

Quantity

1

Base Price: ₹ 27,367.50

GST (18%): ₹ 4,926.15

Total Price: ₹ 32,293.65

Antigen

IgG1

Classification

Monoclonal

Concentration

0.2mg/mL

Description

Ighg1; Immunoglobulin heavy constant gamma 1; Igh-4

Host Species

Rat

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Mouse

Isotype

IgG1 κ

Applications

Flow Cytometry

Clone

A85-1

Conjugate

Brilliant Violet 711

Formulation

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

Immunogen

Pooled IgG1

Quantity

50 μg

Primary or Secondary

Secondary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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Description

  • The A85-1 antibody reacts specifically with mouse IgG1 of Igh-Ca and Igh-Cb haplotypes
  • It does not react with other Ig isotypes
  • Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with the A85-1 monoclonal antibody
  • A suspension of pooled mouse IgG1 was used as the source of immunogen
  • The antibody was conjugated to BD Horizon BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
  • This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
  • BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
  • Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
  • However, the spillover can be corrected through compensation as with any other dye combination.