BV480 CD25 Rat anti-Mouse, Brilliant Violet 480, Clone: PC61, BD

Description

• The PC61 monoclonal antibody specifically binds to CD25, the low-affinity IL-2 Receptorαchain (IL-2Rα, p55) expressed on activated T and B lymphocytes from all mouse strains tested
• IL-2Rαby itself is not a signaling receptor
• However, it can combine with IL-2 Receptorβ(CD122) andγc (CD132) chains to form high-affinity, signaling receptor complexes for IL-2
• Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Rα
• CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells
• It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus
• Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance
• It has also been reported that dendritic cells express CD25, recognized by mAb 7D4
• The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 and 7D4
• It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25
• The antibody was conjugated to BD Horizon BV480 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 436nm and Em Max at 478nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon BV510 (525/40nm) filter set
• BV480 has less spillover into the BV605 detector and, in general, is brighter than BV510
• BD Horizon™ BV480 is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 436nm and Em Max at 478nm, BD Horizon BV480 can be excited by the violet laser and detected in the BD Horizon™ BV510 (525/40nm) filter set
• BV480 has less spillover into the BD Horizon™ BV605 detector and, in general, is brighter than BD Horizon BV510.  Due to similar excitation and emission properties, BV480, BV510 and V500 cannot be used simultaneously.