PDI Mouse, Unlabeled, Clone: 34, BD

Description

• The ER is the site of translation of membrane and secretory proteins
• Following synthesis, it shuttles these proteins through a contiguous membrane system to their appropriate destinations
• Protein disulfide isomerase (PDI) is an abundant, multifunctional, eukaryotic protein
• Although it exhibits ubiquitous expression, it is primarily located in the ER lumen
• Here, it functions to catalyze the isomerization of intramolecular disulfide bridges, thereby allowing them to generate their most thermodynamically stable configurations
• This role in rearrangement has lead to the classification of PDI as a chaperone
• Although protein folding occurs in its absence, PDI may be essential for it to proceed at a physiological relevant rate
• In addition, PDI is the β-subunit of prolyl 4- hydroxylase and is a component of the triglyceride transfer complex
• PDI is retained in the ER lumen via its C-terminal -KdaEL sequence
• Via this sequence, it is continuously recycled back to the ER from other membranous compartments
• Thus, PDI is a diverse protein whose primary function may be to correct disulfide bonding and, thus, ensure the most stable conformation of newly synthesized proteins
• Immunofluorescence, Western Blotting