BDB612796
CD11c Hamster anti-Murine, Brilliant Ultraviolet 737, Clone: HL3, BD Horizon™
Manufacturer: BD Biosciences
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BDB612796-Each-of-1 | In Stock | ₹ 29,681.50 |
BDB612796 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 29,681.50
GST (18%): ₹ 5,342.67
Total Price: ₹ 35,024.17
Antigen
CD11c
Classification
Monoclonal
Concentration
0.2mg/mL
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Immunogen
C57BL/6 Mouse Intestinal Intraepithelial Lymphocytes
Quantity
50 μg
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Applications
Flow Cytometry
Clone
HL3
Conjugate
Brilliant Ultraviolet 737
Host Species
Hamster
Purification Method
Affinity Purified
Regulatory Status
RUO
Target Species
Mouse
Isotype
IgG1 λ2
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Description
- The HL3 monoclonal antibody specifically binds to the integrin α x chain of gp150, 95 (CD11c/CD18)
- CD11c is expressed on dendritic cells, CD4- CD8+ intestinal intraepithelial lymphocytes (IEL) and some NK cells
- It is upregulated on IEL and lymph-node T cells following in vivo activation
- Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c
- CD11c plays a role in binding of iC3b
- The antibody was conjugated to BD Horizon BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
- This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm
- BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter
- Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter)
- Due to spectral differences between labeled cells and beads, using BD™ CompBeads can result in incorrect spillover values when used with BD Horizon BUV737 reagents
- Therefore, the use of BD CompBeads or BD CompBeads Plus to determine spillover values for these reagents is not recommended
- Different BUV737 reagents (e.g., CD4 vs
- CD45) can have slightly different fluorescence spillover therefore, it may also be necessary to use clone specific compensation controls when using these reagents.