CD122 Rat anti-Mouse, Brilliant Violet 605, Clone: TM-Beta 1, BD Optibuild™

Description

• The TM- β 1 antibody reacts with the 90-100-kDa β chain shared by the IL-2 and IL-15 receptors (IL-2R β , CD122)
• In the periphery, CD122 is expressed on CD8+ T lymphocytes, NK cells, NK-T cells, dendritic epidermal T cells, subsets of intraepithelial lymphocytes, and macrophages
• Small subsets of fetal and adult thymocytes constitutively express CD122
• CD122+ cells in the bone marrow include committed NK-cell progenitors
• IL-2R β expression is upregulated by IL-2
• CD122 is a transmembrane glycoprotein of the hematopoietin receptor superfamily which can combine with CD132 ( γc) alone or CD132 plus CD25 (IL-2R α ) to form intermediate or high-affinity IL-2 receptor complexes, respectively
• The β chain of these complexes, CD122, is involved in signal transduction and immunoregulation
• The TM- β 1 antibody blocks high affinity binding of IL-2 or IL-15 to IL-2R β
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.