CD122 Rat anti-Mouse, Brilliant Violet 650, Clone: TM-Beta 1, BD Optibuild™

Description

• The TM- β 1 antibody reacts with the 90-100-kDa β chain shared by the IL-2 and IL-15 receptors (IL-2R β , CD122)
• In the periphery, CD122 is expressed on CD8+ T lymphocytes, NK cells, NK-T cells, dendritic epidermal T cells, subsets of intraepithelial lymphocytes, and macrophages
• Small subsets of fetal and adult thymocytes constitutively express CD122
• CD122+ cells in the bone marrow include committed NK-cell progenitors
• IL-2R β expression is upregulated by IL-2
• CD122 is a transmembrane glycoprotein of the hematopoietin receptor superfamily which can combine with CD132 ( γc) alone or CD132 plus CD25 (IL-2R α ) to form intermediate or high-affinity IL-2 receptor complexes, respectively
• The β chain of these complexes, CD122, is involved in signal transduction and immunoregulation
• The TM- β 1 antibody blocks high affinity binding of IL-2 or IL-15 to IL-2R β
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.