CD2 Rat anti-Mouse, Brilliant Violet 650, Clone: RM2-5, BD Optibuild™

Description

• The RM2-5 monoclonal antibody specifically binds to the immunoglobulin superfamily adhesion molecule, CD2 (LFA-2)
• CD2 is a type I transmembrane glycoprotein that serves as the the major receptor for CD48 in the mouse
• CD2 is involved in T-cell activation, immunoregulation, and thymocyte maturation
• In the mouse, CD2 is expressed on peripheral T lymphocytes, B lymphocytes, and NK cells, and a subpopulation of intraepithelial T lymphocytes
• CD2 is expressed throughout mouse thymic ontogeny, except for distinct subsets of the CD4-CD8- early thymocytes
• In the mouse bone marrow, CD2 is expressed on B220+ sIg+ CD43- pre-B cells, but not on CD43+ pro-B cells
• The RM2-5 antibody is one of a set of five anti-mouse CD2 monoclonal antibodies that were classified into two groups according to their mutual competition in binding to cell surface CD2 and which block CD2-mediated cell-cell adhesion
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.