CD69 Hamster anti-Mouse, Brilliant Violet 650, Clone: H1.2F3, BD Optibuild™

Description

• The H1.2F3 monoclonal antibody specifically binds to CD69 (Very Early Activation antigen), an 85 kDa disulfide-linked homodimer of differentially glycosylated subunits
• CD69 is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules
• Its expression is rapidly induced upon activation of lymphocytes (T, B, NK, and NK-T cells), neutrophils, and macrophages
• CD69 is expressed also on thymocytes that are undergoing positive selection; its role in that process is unclear
• H1.2F3 mAb augments PMA-induced T-cell stimulation and IFN- γ-induced macrophage stimulation
• IL-2-activated NK cells express CD69, and H1.2F3 mAb induces redirected lysis of FcR-bearing target cells by NK cells
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.