CD31 Rat anti-Mouse, Brilliant Violet 650, Clone: 390, BD Optibuild™

Description

• The 390 monoclonal antibody specifically binds to CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule)
• CD31 is a ∽130 kDa integral membrane glycoprotein, a member of the immunoglobulin superfamily, that mediates homophilic and heterophilic cell-cell adhesion
• CD31 is expressed constitutively on the surface of adult and embryonic endothelial cells and is weakly expressed on many peripheral leukocytes and platelets
• It has also been detected on bone marrow-derived hematopoietic stem cells and embryonic stem cells
• CD31 is involved in the transendothelial emigration of neutrophils, and neutrophil PECAM-1 appears to be down-regulated after extravasation into inflamed tissues
• Multiple alternatively spliced isoforms are detected during early post-implantation embryonic development; this alternative splicing is involved in regulation of ligand specificity
• CD38 and vitronectin receptor ( α v β 3 integrin, CD51/CD61) are proposed to be ligands for CD31
• CD31-mediated endothelial cell-cell interactions are involved in angiogenesis
• The 390 mAb inhibits a variety of in vitro and in vivo functions mediated by CD31
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.