CD180 Rat anti-Mouse, Brilliant Violet 650, Clone: RP/14, BD Optibuild™

Description

• The RP/14 monoclonal antibody specifically binds to CD180, the “radioprotective” 105 kDa (RP105) antigen expressed on mature B lymphocytes
• Like other members of the Toll-like Receptor (TLR) family, the extracellular region of CD180 contains tandem repeats of a leucine-rich motif flanked by regions containing conserved cysteines
• These characteristic structural components of TLR may mediate protein-protein interactions and regulation of signal transduction involved in innate immune responses
• Furthermore, CD180 associates with the secretory protein MD-1 on the cell surface
• Cross-linking of cell-surface CD180 with the RP/14 antibody induces activation signals in B lymphocytes
• Despite the expression of CD180 on lymphocytes of all strains tested (BALB/c, BALB/c
• xid , C3H, C57BL/6, CBA/N, and 129/Sv), some strains (BALB/c
• xid , C57BL/6
• xid , CBA/N, 129/Sv, and 129/Ola) display deficient responses to that stimulatory effects of mAb RP/14
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.