CD94 Rat anti-Mouse, Brilliant Violet 711, Clone: 18d3, BD Optibuild™

Description

• The 18d3 monoclonal antibody specifically recognizes CD94 on all NK cells, NK1.1- or DX5-positive T lymphocytes (NK-T cells), and a subset of CD8-positive T lymphocytes in most strains tested (eg, A/J, AKR/J, BALB/c, C3H/He, C57BL/6, CBA/J, DBA/1, FVB/N, 129/Sv, NOD, SWR, and most DBA/2 substrains, but not DBA/2J)
• DBA/2J mice do not express CD94.3 CD94 is also expressed on CD8+ T lymphocytes activated in vivo
• CD94 is a type-II transmembrane protein with an extracellular lectin-like domain and a short cytoplasmic tail which is not believed to have any signalling function
• Heterodimers of CD94 with NKG2A, NKG2C, or NKG2E recognize Qa-1 (a non-classical MHC class I antigen) presenting the Qdm peptide
• Studies on CD94/NKG2 heterodimers on human NK cells have demonstrated that the NKG2 components mediate signal transduction for the receptor, NKG2A being inhibitory and NKG2C being stimulatory
• Similarly, the mouse NKG2A molecule contains two intracytoplasmic sequences which resemble the ITIM ( Immunoreceptor Tyrosine-based Inhibitory Motif ) consensus sequence
• CD94/NKG2 receptors appear on fetal NK cells before the Ly- 49 MHC class I receptors, suggesting that CD94/NKG2 receptors and their ligand, Qa-1, may play a role in maintenance of self-tolerance in developing NK cells
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.