PSEN2 Monoclonal Antibody (APS 26), Invitrogen™

Description

• MA1-754 detects presenilin 2 protein (PS2) from rat, mouse, human, and nonhuman primate samples
• No cross-reactivity is seen with presenilin 1
• MA1-754 has successfully been used in immuno-fluorescence, immunocytochemistry, Western blot and ELISA procedures
• By Western blot, this antibody detects an ∽20 kDa protein representing PS2 CT (C-terminal fragment) and the ∽45 kDa full-length PS2 from transfected COS-7 cells
• In 4% paraformaldehyde fixed RAW cells, MA1-754 showed specific Golgi and ER labeling
• The MA1-754 immunogen is a synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2
• Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP)
• These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains)
• Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP
• Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes
• Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified.