Calprotectin (S100A9) Monoclonal Antibody (MAC387), FITC, Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MA180263-Each-of-1 | In Stock | ₹ 50,329.50 |
MA180263 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 50,329.50
GST (18%): ₹ 9,059.31
Total Price: ₹ 59,388.81
Antigen
Calprotectin (S100A9)
Classification
Monoclonal
Concentration
0.1 mg/mL
Formulation
PBS with 1% BSA and 0.09% sodium azide
Gene Accession No.
P05109, P06702, P28782, P28783, P50115, P50116, P50117
Gene Symbols
S100A8, S100A9
Immunogen
Human monocytes.
Quantity
100 μg
Primary or Secondary
Primary
Target Species
Bovine, Canine, Deer, Equine, Feline, Goat, Guinea Pig, Human, Non-human Primate, Porcine, Rabbit, Rat
Product Type
Antibody
Isotype
IgG1
Applications
Flow Cytometry
Clone
MAC387
Conjugate
FITC
Gene
S100A9
Gene Alias
60B8Ag; AI323541; AW546964; B8Ag; BEE11; BEE22; CAGA; CAGB; calcium binding protein; S100 protein; calgranulin A; calgranulin B; calgranulin-A; Calgranulin-B; calprotectin; calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; CGLA; CGLB; chemotactic cytokine CP-10; chemotactic S100 protein; CP-10; Cystic fibrosis antigen; EGK_01322; EGK_21438; GAGB; intracellular calcium-binding protein (MRP14); L1AG; Leukocyte L1 complex heavy chain; leukocyte L1 complex light chain; LIAG; LOC100735514; LOC532569 protein; MA387; MAC387; macrophage migration inhibitory factor-related protein-14; macrophage migration inhibitory factor-related protein-8; MIF; migration inhibitory factor-related protein 14; Migration inhibitory factor-related protein 8; Mrp14; MRP-14; MRP8; MRP-8; myeloid-related protein 14; Neutrophil cytosolic 23 kDa protein; neutrophil cytosolic 7 kDa protein; NIF; p14; p23; P7; p8; Pro-inflammatory S100 cytokine; protein S100-A8; Protein S100-A9; S100 calcium binding protein A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; protein S100-A9; S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100a8; S100A8/S100A9; S100a9; similar to Calgranulin A (Migration inhibitory factor-related protein 8) (MRP-8) (Cystic fibrosis antigen) (CFAG) (P8) (Leukocyte L1 complex light chain) (S100 calcium-binding protein A8) (Calprotectin L1L subunit) (Urinary stone protein band A)...; urinary stone protein band A
Host Species
Mouse
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
100008704, 100008985, 100061730, 100061766, 100127488, 100127489, 100735238, 100735514, 101084164, 101084419, 102168876, 102182273, 116547, 490461, 490463, 532569, 616818, 6279, 6280, 94195
Content And Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Form
Liquid
Related Products
Description
- This clone recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain
- Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al
- 1987) subsequent studies indicate that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al
- 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets
- In addition Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha
- The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages
- Variable results have been reported for staining brain macrophages and microglia
- The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cellds in a wide range of species
- Membrane permeabilization is required for flow cytometry applications
- Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL
- The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils
- Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly
- The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects
- Besides this it is a potent chemotactic factor for neutrophils
- Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease
- Granulocytes terminate their existence after transmigration through the intestinal wall
- Therefore calprotectin is also detectable in feces
- Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.