mtHSP70 Monoclonal Antibody (JG1), Invitrogen™
Manufacturer: Thermo Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MA3028-Each-of-1 | In Stock | ₹ 47,659.50 |
MA3028 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,659.50
GST (18%): ₹ 8,578.71
Total Price: ₹ 56,238.21
Antigen
mtHSP70
Classification
Monoclonal
Concentration
1.07 mg/mL
Formulation
PBS with 1mg/mL BSA and 0.05% sodium azide
Gene Accession No.
P0DMV8, Q61696
Gene Symbols
HSPA1A
Immunogen
Synthetic peptide corresponding to residues G(615) S G S S G T G E Q K E D Q K E E K Q(633) of mouse mtHSP70.
Quantity
100 μL
Primary or Secondary
Primary
Target Species
Canine, Human, Mouse, Non-human Primate
Product Type
Antibody
Isotype
IgG3
Applications
Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot
Clone
JG1
Conjugate
Unconjugated
Gene
HSPA1A
Gene Alias
68 kDa heat shock protein; dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; Heat shock 70 kDa protein 3; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock protein 1A; heat shock protein family A (Hsp70) member 1A; heat shock protein, 70 kDa 3; heat shock-induced protein; HEL-S-103; Hsp68; HSP70.1; HSP70.1/HSP70.2; Hsp70.3; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; Hsp70-3; Hsp70A1; HSP70I; Hsp72; HSPA1; Hspa1a; HSPA1B; HSX70; inducible heat shock protein 70
Host Species
Mouse
Purification Method
Protein A
Regulatory Status
RUO
Gene ID (Entrez)
193740, 3303
Content And Storage
-20° C, Avoid Freeze/Thaw Cycles
Form
Liquid
Related Products
Description
- MA3-028 detects mitochondrial heat shock protein 70 kDa (mtHSP70) from human, non-human primate, canine and mouse tissues
- MA3-028 has been successfully used in Western blot, immunocytochemical, immunofluorescence, immunohistochemical (paraffin), and immunoprecipitation procedures
- By Western blot, this antibody detects a single ∽75 kDa band representing mtHSP70 from U2OS cell homogenate
- Immunocytochemical staining of mtHSP70 in DAP.3 cells with MA3-028 results in a worm-like staining pattern, consistent with mitochondrial localization
- Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit
- This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays
- It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications
- Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay
- The HSP70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined
- The human HSP70 family members include: HSP70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP72, a 72 kDa protein which is induced exclusively under stress conditions; HSC70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP70 (mtHSP70, GRP75 or mortalin) a 75 kDa protein that is found within the mitochondria
- mtHSP70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria
- Preproteins cross the mitochondrial membranes in an extended conformation
- This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane
- Preprotein unfolding is thought to be mediated by mtHSP70 and other inner membrane proteins of the mitochondria.