MilliporeSigma™ Glycoprotein 78, Rat, Unlabeled, Clone: 3F3A,
Manufacturer: MilliporeSigma™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABC949-Each-of-1 | In Stock | ₹ 42,967.42 |
MABC949 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 42,967.42
GST (18%): ₹ 7,734.136
Total Price: ₹ 50,701.556
Antigen
Glycoprotein 78
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified rat IgM in buffer containing PBS without azide.
Gene Accession No.
Q9UKV5
Immunogen
Peanut lectin (PNA) resin-purified membrane glycoprotein 78 from B16-F1 cells (Nabi, I.R., and Raz, A. (1987). Int. J. Cancer. 40(3):396-402).
Quantity
200 μL
Research Discipline
Apoptosis & Cancer
Gene ID (Entrez)
NP_001135
Target Species
Human
Form
Purified
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Clone
3F3A
Conjugate
Unconjugated
Gene
RNF45, AMFR
Host Species
Rat
Purification Method
Purified by Ion-Exchange Chromatography
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone 3F3A detected glycoprotein 78 (gp78) and a ∽150 kDa glycoprotein in B16-F1 mouse melanoma cell extract. Cell extract sialidase treatment decreased the antibody's immunoreactivity toward gp78 and abolished ∽150 kDa band detection (Nabi, I.R., et al. (1990). Cancer Res. 50(2):409-414).
Content And Storage
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype
IgM κ
Related Products
Description
- E3 ubiquitin-protein ligase AMFR (UniProt Q9UKV5; also known as AMF receptor, Autocrine motility factor receptor, gp78, RING finger protein 45) is encoded by the RNF45 (also known as AMFR) gene (Gene ID 267) in human
- Glycoprotein 78 (gp78) is an endoplasmic reticulum (ER) membrane-anchored E3 ubiquitin ligase that plays a key role in ER-associated degradation (ERAD)
- Gp78 is localized to a mitochondria-associated ER domain, where it induces mitochondrial fragmentation by targeting the mitochondrial fusion proteins mitofusin 1 and 2 (Mfn1 and Mfn2, respectively) for degradation upon mitochondrial depolarization
- Other known ERAD substrates processed by gp78 include ApoB lipoprotein, HMG CoA reductase, CD3-delta, cystic fibrosis transmembrane conductance regulator, the metastasis suppressor KAI1, unglycosylated prion protein (PrP), and the T-cell receptor
- Gp78 is a 7-transmembrane protein (a.a
- 82-102, 122-142, 145-165, 186-206, 215-235, 276-296, 429-449) with a RING-type Zinc finger (a.a
- 341-379), a CUE domain (a.a
- 456-498), and a VCP/p97-interacting motif (VIM; a.a
- 622-640).