Chlamydial LPS, Mouse anti-Bacteria, Clone: EVI-H1, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABF210725-Each-of-1 | In Stock | ₹ 17,118.26 |
MABF210725 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 17,118.26
GST (18%): ₹ 3,081.287
Total Price: ₹ 20,199.547
Antigen
Chlamydial LPS
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Bacteria
Form
Purified
Applications
Immunocytochemistry, Immunofluorescence, Immunomicroscopy, Western Blot
Clone
EVI-H1
Dilution
Immunofluorescence Analysis: A representative lot detected Chlamydial LPS in Immunofluorescence applications (Su, H., et. al. (2000). Infect Immun. 68(1):192-6; Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Immunocytochemistry Analysis: A representative lot detected Chlamydial LPS in Immunocytochemistry applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).Electron Microscopy Analysis: A representative lot detected Chlamydial LPS with Trasnsmission Electron Microscopy applications (Wolf, K., et. al. (2001). Infect Immun. 69(5):3082-91).
Gene Alias
Chlamydial lipopolysaccharide;Chlamydia trachomatis LPS
Immunogen
Formalin-killed elementary bodies of the Chlamydia trachomatis L2 serovar.
Quantity
25 μg
Research Discipline
Inflammation & Immunology
Test Specificity
Clone EVH-H1 detects lipopolysaccharide from Chlamydia genus.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG2a
Related Products
Description
- Anti-Chlamydial LPS, clone EVI-H1, Cat
- No
- MABF2107, is a mouse monoclonal antibody that detects Chlamydial lipopolysaccharide (LPS) and has been tested for use in Electron Microscopy, Immunocytochemistry, Immunofluorescence, and Western Blotting
- Chlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV)
- Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants
- These are identified based on serological reactivity of the epitopes on their outer membrane
- Intracellularly chlamydia replicates within a vacuole
- Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles
- This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell
- Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate
- Lipopolysaccharides (LPS) of Chlamydia consists of at least three antigen domains, two of which are shared by the LPS of certain other gram-negative organisms and one that is unique only to chlamydial LPS
- It has been reported that the quantitative yields of chlamydial LPS can be achieved when elementary bodies (EBs) are first reduced and alkylated prior to extraction with hot phenol-water
- (Ref.: Caldwell, HD., and Hitchcock, PJ
- (1984)
- Infect
- Immun
- (44(2); 306-314; Scidmore, MA., et al
- (1996)
- Infect
- Immun
- 64(12); 5366-5372; Turingan, RS et al
- (2017)
- PLoS ONE 12(5): e0178653).