Chlamydial HSP60, Mouse anti-Bacteria, Clone: A57-B9, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABF210825-Each-of-1 | In Stock | ₹ 16,643.00 |
MABF210825 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,643.00
GST (18%): ₹ 2,995.74
Total Price: ₹ 19,638.74
Antigen
Chlamydial HSP60
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
N/A
Immunogen
Recombinant Chlamydia trachomatis serovar A HSP60 emulsified in complete Freund adjuvant.
Quantity
25 μg
Research Discipline
Inflammation & Immunology
Test Specificity
Clone A57-B9 detects Chlamydial HSP60. It targets an epitope with in 144 amino acids in the carboxy terminal of HSP60.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Clone
A57-B9
Dilution
Immunocytochemistry Analysis: A representative lot detected Chlamydial HSP60 in Immunocytochemistry applications (Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).Immunofluorescence Analysis: A representative lot detected Chlamydial HSP60 in Immunofluorescence applications (Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9).ELISA Analysis: A representative lot detected Chlamydial HSP60 in ELISA applications (Morrison, S.G., et. al. (2005). J Immunol. 175(11):7536-42).Western Blotting Analysis: A representative lot detected chlamydial HSP60 in Western Blotting applications (Hechard, C., et. al. (2004). J Med Microbiol. 53(Pt 9):861-8; LaVerda, D., et. al. (1997). J Clin Microbiol. 35(5):1209-15; Southern, T., et. al. (2012). Clin Vaccine Immunol. 19(11):1864-9; Yuan, Y., et. al. (1992). Infect Immun. 60(6):2288-96).
Gene Alias
Chlamydia heat shock protein 60
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
Bacteria
Form
Purified
Related Products
Description
- Anti-Chlamydial HSP60, clone A57-B9, Cat
- No
- MABF2108, is a mouse monoclonal antibody that detects Chlamydial HSP60 and has been tested for use in ELISA, Immunofluorescence, Immunocytochemistry, and Western Blotting
- Chlamydia trachomatis is a Gram-negative bacterium that is responsible to sexually transmitted diseases leading to pelvic inflammatory disease, ectopic pregnancy, infertility, and outbreaks of trachoma-associated blindness and lymphogranuloma venereum (LGV)
- Chlamydia trachomatis consists of eighteen different serological variants (serovars) that include a few subvariants
- These are identified based on serological reactivity of the epitopes on their outer membrane
- Intracellularly chlamydia replicates within a vacuole
- Chlamydia infection is initiated with the expression of a chlamydial early gene product(s), which isolate the inclusion from the endocytic-lysosomal pathway and makes it fusogenic with sphingomyelin-containing exocytic vesicles
- This change in vesicular interaction allows the delivery of the vacuole to the peri-Golgi region of the host cell
- Antigens from all members of the Chlamydia genus display heat resistance and sensitivity to oxidation by sodium periodate
- Clone A57-B9 detects Chlamydial HSP60 (GroEL)
- It reacts with the short peptide of 6 amino acids from the carboxyl terminal region of HSP60
- HSP60 facilitates the correct folding of imported proteins and may prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions.